바이오이미징을 위한 ultrafast laser

Spectra-Physics는 바이오 이미징 분야에 수 천대의 ultrafast 레이저를 판매한 NO.1 제조사로써, 다광자 및 멀티모듈 이미징 개발을 위한 다년간의 운용으로 쌓은 최고의 신뢰성을 바탕으로 하는 선도기업입니다.

쥐 뇌피질의 다색(혹은 멀티 컬러) 3D 이미지

바이오이미징을 위한 ultrafast laser 제품군

제품설명파장펄스 폭파장변환기
파장 가변 초고속레이저  
new
InSight X3
멀티포톤 이미징용 파장가변형 Ultrafast 레이저 시스템
 
Wavelength
680–1300 nm
Pulse Width
<120 fs
Wavelength Converters
N/A
 
Mai Tai DeepSee
폭넓은 가변범위와 최고출력을 위한 분산 보상 장치가 내장
 
Wavelength
690–1040 nm
710–920 nm
710–950 nm
710–990 nm
Pulse Width
<70, 100 fs
<80 fs
<80 fs
<70 fs
Wavelength Converters
 
Mai Tai
폭넓은 가변범위와 극초단 펄스 폭
 
Wavelength
690–1040 nm
710–920 nm
710–950 nm
710–990 nm
Pulse Width
<100 fs
<80 fs
<80 fs
<70 fs
 
고정 파장폭 초고속 레이저  
new
Spirit
초정밀 미세가공의 새로운 표준을 제시해 드립니다
 
Wavelength
515 nm
520 nm
1030 nm
1040 nm
Pulse Width
<400 fs
Wavelength Converters
 
HighQ-2
초소형 턴키 방식의 단일 파장의 펨토초 레이저
 
Wavelength
522, 1045 nm
Pulse Width
<250 fs
Wavelength Converters
Built in
 
femtoTrain
소형 턴키 방식의 높은 에너지 펨토초 레이저
 
Wavelength
1040 nm
Pulse Width
<220 fs
Wavelength Converters
N/A
 

바이오 이미징을 위한 초고속 레이저 활용

Mouse brain, hippocampus region; imaged with femtoTrain™.
Courtesy of Sabine Scheibe, LMU Munich and Tilman Franke, FEI Munich GmbH



Mouse barrel cortex, after clearing.
Courtesy of Gabriel Jones, Steven Petrou, University of Melbourne, Australia



Hippocampus CA3 pyramidal neuron; imaged with InSight® DS+™.
Courtesy of Karina Alvina, Albert Einstein College of Medicine



Multicolor image of live mouse brain; imaged with InSight DS+.
Courtesy of Naoki Honkura and Takeshi Imamura, Ehime University Graduate School of Medicine



Zebrafish embryo brain development, CFP, YFP, mCherry imaged at 860 nm and 1041 nm with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Maximum intensity projection of zebrafish embryo development, taken from both sides over 8 hours; imaged with InSight DS+.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Z-stack of transgenic zebrafish larvae, labeled with GFP and CFP; imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Rachel Wong, University of Washington, Seattle



Transgenic zebrafish embryo retina, revealing various neuronal cell types, imaged at 927 nm and 1041 nm with InSight DS+.
Courtesy of Dr. Xana Almeida, University of Cambridge, UK



Lymph vessel; imaged with InSight® DS+™.
Courtesy of Dr. Cameron Newell, Monash, University, Melbourne, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Mouse ear, showing collagen (blue) and GFP labelled T cells (green); imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Claudio Vinegoni, Mass General Hospital, Harvard University



Retinal ganglion cell; imaged with Mai Tai.
Courtesy of Dr. Gregory Schwartz, Northwestern University



Chemically cleared mouse heart total depth of 3.5 mm; imaged with Mai Tai DeepSee.
Courtesy of Mayandi Sivaguru, University of Illinois Urbana Champaign and Sakthivel Sadayappan, Loyola University, Chicago



Human meibomian gland, CARS imaging the lipid rich meibocytes and SHG visualizing the surrounding collagen;
acquired with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine



SRS image of fatty liver, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



SRS image of spinal cord, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



CARS Z-stack of C.Elegans worm, visualizing lipids 19.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



CARS and MPEF imaging of C. Elegans, neuron cells labelled with GFP, lipid droplets revealed with CARS.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



Zebrafish embryo (6 hours post fertilization) using label free THG at 1140 nm; imaged with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France



Mouse blood vessel wall, SHG and multiphoton excited fluorescence imaging with HighQ-2™.
Courtesy of Dr. Marc van Zandvoort, Maastrich University



Mouse mammary gland, label free image of collagen (SHG, magenta) and adipocytes (THG, yellow), imaged with InSight DS+™.
Courtesy of Dr. Marie Irondelle, Institut Curie/CNRS, Paris, France



3D images from a mouse brain cerebellum extending 1 mm deep into the tissue acquired via 3PF (left)
and THG (right) microscopy at 1.3 μm using a Spirit laser with a Spirit-NOPA.
Courtesy of Chris Xu, with permission from SPIE Publications: Wang, et. al., “In vivo three-photon
imaging of deep cerebellum,” Proc. SPIE:  Multiphoton Microscopy in the Biomedical Sciences
XVIII, vol. 10498, 2018.

Mouse kidney, with CARS (red), SHG (blue) and autofluorescence (green); imaged with Mai Tai® HP plus Inspire™ OPO.
Courtesy of Dr. Eric Potma, UC Irvine, CA



C. elegans, with CARS mapping out lipid rich areas and two-photon excited fluorescence from GFP and dsRed;
imaged with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine, CA